Julia Mahamid

Julia Mahamid develops methods for in-cell structural biology at near-atomic resolution by cryo-electron tomography and combines them with quantitative light microscopy and multi-omics approaches to derive structure-function relations. Her work revealed that LD core neutral lipids undergo structural phase transitions as a function of the cellular metabolic state.

ORCID: 0000-0001-6968-041X

julia.mahamid[at]embl.de

Mahamid lab

Select Publications

Zooming into lipid droplet biology through the lens of electron microscopy. Invited review
Dudka W, Salo VT, Mahamid J
FEBS Letters 598(10):1127-1142 2024
doi: 10.1002/1873-3468.14899 PMID: 38726814

Molecular mechanisms of stress-induced reactivation in mumps virus condensates
Zhang X, Sridharan S, Zagoriy I, Eugster Oegema C, Ching C, Pflaesterer T, Fung KHH, Poser I, Müller C, Hyman AA, Savitski M, Mahamid
Cell 186, 1877-1894 2022
doi:10.1016/j.cell.2023.03.015 PMID: 37116470 PMCID: PMC10156176

Light and electron microscopy continuum resolution imaging of 3D cell cultures
D’Imprima E, Garcia Montero M, Gawrzak S, Ronchi P, Zagoriy I, Schwab Y, Jechlinger M, Mahamid J
Cell 58 616-632 2023
doi: 10.1016/j.devcel.2023.03.001 PMID: 36990090 PMCID: PMC10114294

Convolutional networks for supervised mining of molecular patterns within cellular context
de Teresa-Trueba I, Goetz SK, Mattausch A, Stojanovska F, Zimmerli C, Toro-Nahuelpan M, Cheng DWC, Tollervey F, Pape C, Beck B, Kreshuk A, Mahamid J, Zaugg J
Nature Methods 20, 284–294 2023
doi: 10.1038/s41592-022-01746-2 PMID: 36690741 PMCID: PMC9911354

Visualizing translation dynamics at atomic detail in a bacterial cell
Xue L, Lenz S, Zimmermann-Kogadeeva M, Tegunov D, Cramer P, Bork P, Rappsilber J, Mahamid J
Nature 610, 05–211 2022
doi: 10.1038/s41586-022-05255-2 PMID: 36171285 PMCID: PMC9534751

A Modular Platform for Automated Cryo-FIB Workflows
Klumpe S*, Fung HKH, Goetz SK, Zagoriy I, Hampoelz B, Zhang X, Erdmann PS, Baumbach J, Müller CW, Beck M, Plitzko JM, Mahamid J
eLife 10:e70506 2021
doi: 10.7554/eLife.70506 PMID: 34951584 PMCID: PMC8769651

Multi-particle cryo-EM refinement with M visualizes ribosome-antibiotic complex at 3.5 Å inside cells
Tegunov D, Xue L, Dienemann C, Cramer P, Mahamid J
Nature Methods 18, 186–193 2021
doi: 10.1038/s41592-020-01054-7 PMID: 33542511 PMCID: PMC7611018

In-cell architecture of an actively transcribing-translating expressome
O’Reilly F, Xue L, Graziadei A, Sinn L, Lenz S, Tegunov D, Blötz C, Hagen WJH, Cramer P, Stülke J, Mahamid J, Rappsilber J
Science 369, 554-557 2020
doi: 10.1126/science.abb3758 PMID: 32732422 PMCID: PMC7115962

Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies
Toro-Nahuelpan M, Zagoriy I, Senger F, Blanchoin L, Théry M, Mahamid J
Nature Methods 17, 50-54 2020
doi: 10.1038/s41592-019-0630-5 PMID: 31740821 PMCID: PMC6949126

Liquid-crystalline phase transitions in lipid droplets are related to cellular states and specific organelle association
Mahamid J, Tegunov D, Maiser A, Arnold J, Leonhardt H, Plitzko, JM, Baumeister W
* Proceedings of the National Academy of Sciences USA* 116, 16866-16871 2019
doi: 10.1073/pnas.1903642116 PMID: 31375636 PMCID: PMC6708344

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